Evolution of egg size and fertilisation efficiency in sea stars: large eggs are not fertilised more readily than small eggs in Patiriella (Echinodermata: Asteroidea)

Fertilisation kinetics theory suggests that, when sperm are limiting, the larger eggs of broadcast-spawning marine organisms ought to be fertilised more frequently than smaller eggs, because they provide a bigger target for searching sperm. Whilst this effect has been demonstrated within species, it is not known if this pattern holds among species. We tested whether a large difference in egg size between congeneric seastars with contrasting planktotrophic and lecithotrophic modes of development results in differences in the likelihood of eggs being fertilised in sperm-limiting situations. Measurement of egg sizes and sperm swimming speeds led to the prediction that the sperm–egg collision rate constant for Patiriella calcar (420-µm-diameter egg) should be nine times greater than for P. regularis (140-µm-diameter egg). Although the eggs of P. calcar should be fertilised at greater rates in low sperm concentrations, they were not. When gametes were allowed to mix for 10 s, the hypothesis that P. calcar eggs required less sperm than P. regularis to ensure 50% of eggs were fertilised was rejected. When gametes were mixed for 5 min, P. regularis eggs were more frequently fertilised, but the difference was not statistically significant. We conclude there must be a difference between these species in the likelihood that when a sperm finds a conspecific egg it can successfully fertilise. This apparent uncoupling of egg size and likelihood of fertilisation suggests that fertilisation is not a major constraint on the evolution of egg size in these seastars.

Can oiled shorebirds and their nests and eggs be successfully rehabilitated? A case study involving the threatened Hooded plover Thinornis rubricollis in South-eastern Australia

Although shorebirds are detrimentally affected by marine oil spills, they are often overlooked during rescue and rehabilitation efforts. This note describes a rescue and successful rehabilitation effort of an oiled adult and a juvenile Hooded Plover (Thinornis rubricollis) in Victoria, south-eastern Australia, during an oil-spill which oiled almost 1% of the State’s population of this threatened beach-nesting species. Two birds requiring intervention were located, selectively captured, cleaned and released. Both have survived at least two years after the spill and have bred, with at least one successfully fledging young. The fledgling has also successfully bred. Two nests with eggs present during clean-up operations were protected and hatched successfully. This small case study indicates that at least some groups of breeding shorebirds, such as plovers and dotterels, can be effectively rescued and rehabilitated during oil spills, and hence should not be overlooked during such circumstances.

Conditioned taste aversion reduces fox depredation on model eggs on beaches

Context:  Conditioned taste aversion (CTA) is induced by an association of a food item with a negative experience, such as illness, which causes animals to avoid subsequent consumption of that particular food item. Inducing CTA may help reduce depredation rates of threatened fauna where predator population control is undesirable, impractical or unsuccessful.

Aims:  We investigated whether CTA could be induced among foxes (Vulpes vulpes) to model eggs which mimicked those of the threatened hooded plover (Thinornis rubricollis).

Methods:   Model eggs treated with a potential CTA-inducing chemical (sodium carbonate) and control eggs free of the agent were exposed to fox depredation for 28 days to simulate a hooded plover incubation period. To investigate whether CTA would persist in wild foxes, we implemented a part-time agent treatment (an initial 14 day exposure period of model eggs with the CTA agent followed by a second 14 day period when model eggs were free of the agent).

Key results:  Similar intervals to the first depredation event were found for all model eggs regardless of treatment. After the first depredation event by foxes, the rate and likelihood of fox depredation was significantly lower in treated eggs than in control eggs. The likelihood or rate of depredation across the three treatments did not differ between the first and second periods.

Conclusions: Our results suggest that during an exposure period of at least 28 days, CTA can be induced in wild foxes to eggs on beaches. Our results also suggest that 14 days may be insufficient time for wild foxes to develop a lasting CTA to familiar food items such as eggs.

Implications:  Treatment of eggs with a CTA-inducing chemical may present a viable alternative to traditional predator control techniques for hooded plovers, as well as other ground-nesting birds, provided that an extended exposure to the CTAinducing agent occurs.

Extracting DNA from museum bird eggs, and whole genome amplification of archive DNA

We present a comprehensive protocol for extracting DNA from egg membranes and other internal debris recovered from the interior of blown museum bird eggs. A variety of commercially available DNA extraction methods were found to be applicable. DNA sequencing of polymerase chain reaction (PCR) products for a 176-bp fragment of mitochondrial DNA was successful for most egg samples (> 78%) even though the amount of DNA extracted (mean = 14.71 ± 4.55 ng/µL) was significantly less than that obtained for bird skin samples (mean = 67.88 ± 4.77 ng/µL). For PCR and sequencing of snipe (Gallinago) DNA, we provide eight new primers for the ‘DNA barcode’ region of COI mtDNA. In various combinations, the primers target a range of PCR products sized from 72 bp to the full ‘barcode’ of 751 bp. Not all possible combinations were tested with archive snipe DNA, but we found a significantly better success rate of PCR amplification for a shorter 176-bp target compared with a larger 288-bp fragment (67% vs. 39%). Finally, we explored the feasibility of whole genome amplification (WGA) for extending the use of archive DNA in PCR and sequencing applications. Of two WGA approaches, a PCR-based method was found to be able to amplify whole genomic DNA from archive skins and eggs from museum bird collections. After WGA, significantly more archive egg samples produced visible PCR products on agarose (56.9% before WGA vs. 79.0% after WGA). However, overall sequencing success did not improve significantly (78.8% compared with 83.0%).

Production and characterization of hypoallergenic chicken eggs

This project aimed to develop novel therapeutics and methods to tackle egg allergy. In this study, recombinant variants of egg allergens were produced that may be used in future immunotherapy. Genetic engineering was also used to develop new strategies to produce non-allergenic chicken eggs.

Intense predation of non-colonial, ground-nesting bird eggs by corvid and mammalian predators

Context Loss of eggs to predators is a major cause of reproductive failure among birds. It is especially pronounced among ground-nesting birds because their eggs are accessible to a wide range of predators. Few studies document the main causes of clutch fate of ground-nesting birds. Aims The main objective of the present study was to identify the major egg predator of red-capped plovers (Charadrius ruficapillus). We also investigated the effectiveness of the following two primary strategies available to the plovers to avoid egg predation: (1) the placement of clutches under vegetative cover and (2) avoiding predators by nesting outside the peak season of predator occurrence. Methods Remote-sensing cameras were deployed on plover nests to identify egg predators and nests were monitored over four breeding seasons to document reproductive success and fate. An experiment using false clutches with model eggs investigated the influence of nest cover on the risk of egg predation throughout the year. Line-transect surveys were conducted to estimate the abundance of egg predators in and around the wetlands. Key results The little raven (Corvus mellori) was the major egg predator identified in 78.6% of red-capped plover clutches and in 92.4% of false clutches that were camera-monitored. The hatching success of plover eggs was not influenced by nest cover (P≤0.36), but model egg survival in false clutches improved significantly with the presence of nest cover (P≤0.02). The abundance of little ravens increased during the plover breeding season and was highly negatively correlated with false clutch survival (rpearson≤-0.768, P≤0.005). Conclusions Little ravens were the major predator of red-capped plover eggs and their abundance increased significantly during the plover breeding season. Any influence of nest cover on hatching success of eggs may have been masked by the extremely high rate of egg loss associated with the increased little raven abundance during the plover breeding season. Implications The high rate of egg predation is likely to have negative consequences on the local red-capped plover population, suggesting management is warranted. Little raven populations have expanded and, thus, their impact as egg predators needs to be investigated especially on threatened species. Journal compilation

Out of sight but not out of mind: corvids prey extensively on eggs of burrow-nesting penguins

Context Egg depredation is a major cause of reproductive failure among birds and can drive population declines. In this study we investigate predatory behaviour of a corvid (little raven; Corvus mellori) that has only recently emerged, leading to widespread and intense depredation of eggs of a burrow-nesting seabird (little penguin; Eudyptula minor). Aims The main objective of this study was to measure the rate of penguin egg depredation by ravens to determine potential threat severity. We also examined whether penguin burrow characteristics were associated with the risk of egg depredation. Ravens generally employ two modes of predatory behaviour when attacking penguin nests; thus we examined whether burrow characteristics were associated with these modes of attack. Methods Remote-sensing cameras were deployed on penguin burrows to determine egg predation rates. Burrow measurements, including burrow entrance and tunnel characteristics, were measured at the time of camera deployment. Key results Overall, clutches in 61% of monitored burrows (n≤203) were depredated by ravens, the only predator detected by camera traps. Analysis of burrow characteristics revealed two distinct types of burrows, only one of which was associated with egg depredation by ravens. Clutches depredated by ravens had burrows with wider and higher entrances, thinner soil or vegetation layer above the egg chamber, shorter and curved tunnels and greater areas of bare ground and whitewash near entrances. In addition, 86% were covered by bower spinach (Tetragonia implexicoma), through which ravens could excavate. Ravens used two modes to access the eggs: they attacked through the entrance (25% of burrow attacks, n≤124); or dug a hole through the burrow roof (75% of attacks, n≤124). Burrows that were subject to attack through the entrance had significantly shorter tunnels than burrows accessed through the roof. Conclusions The high rates of clutch loss recorded here highlight the need for population viability analysis of penguins to assess the effect of egg predation on population growth rates. Implications The subterranean foraging niche of a corvid described here may have implications for burrow-nesting species worldwide because many corvid populations are increasing, and they exhibit great capacity to adopt new foraging strategies to exploit novel prey. Journal compilation